Plasmid_Backbone

Part:BBa_K174018:Design

Designed by: The Newcastle 2009 iGEM team   Group: iGEM09_Newcastle   (2009-10-15)


GENEART PMQ Plasmid Backbone


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 420
    Illegal EcoRI site found at 462
    Illegal PstI site found at 2083
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 420
    Illegal EcoRI site found at 462
    Illegal PstI site found at 2083
    Illegal NotI site found at 402
    Illegal NotI site found at 444
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 420
    Illegal EcoRI site found at 462
    Illegal BglII site found at 1715
    Illegal BamHI site found at 412
    Illegal BamHI site found at 454
    Illegal XhoI site found at 394
    Illegal XhoI site found at 436
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 420
    Illegal EcoRI site found at 462
    Illegal PstI site found at 2083
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 420
    Illegal EcoRI site found at 462
    Illegal PstI site found at 2083
    Illegal NgoMIV site found at 1937
    Illegal AgeI site found at 2044
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

We used standard biobrick clonining sites to synthesise our Biobricks. So they should easily be cut with standard restriction enzymes used by iGEM.


Source

GENEART

References